In recent years the interest in the research on lipids (lipidomics) is broadly increased due to the increased knowledge of the crucial role of these molecules in cell, tissue and organ physiology. It's however still difficult to obtain chemical and structure informations due to the complexity of lipids and the lack of powerful tools for their analysis. An important improvement in this direction has been achieved by new analytical approaches such as mass spectrometry and NMR spectroscopy, that make lipidomics a promising field of biomedical research. In this poster we showed some examples from my researches that explained the potential of these techniques and their great complementarity